Police officers have arrived at the farm in Gloucestershire which is home to Geronimo the alpaca.
Uniformed officers could be seen speaking to three people dressed in blue overalls, masks and goggles outside the farm in Wickwar.
It comes just days before the destruction warrant ends on September 4. The alpaca has twice tested positive for bovine tuberculosis, which owner Helen Macdonald believes are false positives.
The destruction warrant is valid until September 4 and Ms Macdonald wants the Department for Environment, Food and Rural Affairs (Defra) to allow Geronimo to be tested for a third time or let him live to aid research into the disease.
A Defra spokesman said: “We are sympathetic to Ms Macdonald’s situation – just as we are with everyone with animals affected by this terrible disease.
“While nobody wants to cull animals, we need to do everything we can tackle this disease to stop it spreading and to protect the livelihoods of those affected.”
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